Voltage Clamp Studies on Internally Perfused Axons
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چکیده
منابع مشابه
Voltage Clamp Studies on Internally Perfused Axons
Probably the most striking and surprising observation in internally perfused axons is that full-sized action potentials can be obtained in the face of a very low resting potential when the ionic strength is low In all experiments at normal ionic strength known to the author, the explosive all-or-none responsiveness was lost when the internal and/or external monovalent cations were arranged to g...
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Methods Isolated and cleaned squid giant axons were internally perfused by a modification of the method of Adelman and Gilbert (1964) and Adelman and Fok (1964). A chemically driven syringe (Sage Instruments) was directly connected to the axon interior through a small cut in one end by means of a 200,/ diameter glass cannula. The internal axoplasm was eroded away upon continuous internal fluid ...
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An improved method for internally perfusing the Myxicola giant axon based on removing the axoplasm by dispersing it in KCl-KF salt solutions is described. Proteolytic enzymes are not introduced. With this improved method perfused preparations show long-term stability of their electrical properties and the ability to generate action potentials for many hours. Mean initial values for resting memb...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1965
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.48.5.11